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| Prizão, Eliane Cristina; Universidade Estadual de Maringá; Gonçalves, Letícia de Menezes; Universidade Estadual de Maringá; Gutierre, Maria Auxiliadora; Universidade Estadual de Maringá; Mangolin, Claudete Aparecida; Universidade Estadual de Maringá; Machado, Maria de Fátima Pires da Silva; Universidade Estadual de Maringá. |
| No previous studies have investigated the use of graphite instead of activated charcoal in orchids. In this work, different concentrations of activated charcoal or graphite were added to KC medium to darken the culture medium and stimulate the in vitro propagation of Cattleya bicolor and of a double hybrid orchid (‘BLC Pastoral Innocence’). The seedlings were inoculated on growth regulator-free KC medium; the effects of activated charcoal (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) and graphite (0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 g L-1) concentrations were tested separately. Each flask contained fifteen seedlings, and the experiments had a randomised, two-way factorial design. The two tested variables were culture medium and illumination levels; eleven culture... |
| Tipo: Laboratory analysis |
Palavras-chave: Activated charcoal; Graphite; Orchids; Micropropagation. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12257 |
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| Demant, Carlos Alberto Rauer; Universidade Estadual Paulista “Julio de Mesquita Filho”; Auld, Dick; Texas Tech University, Lubbock, Texas, United States; Demant, Aline Rodrigues de Mello; Texas Tech University, Lubbock, Texas, United States. |
| In this study, we developed a bioassay to quantify the ricin toxin content of castor bean seeds. Existing quantification methods do not always reflect actual toxicity of the seeds analyzed, which may present lower ricin content even though they are more toxic than seeds presenting a higher content of ricin. This is because these methods actually measure the addition of ricin RCA, which is a compound less toxic than pure ricin. We decided to use in this study, the nematode Caenorhabditis elegans, which has been widely used by the pharmaceutical industry. We tested two strains of C. elegans using different methods in 8 experiments. We examined 4 methods of extracting the ricin complex and 3 methods of exposing the nematodes. Among the nematode strains and... |
| Tipo: Laboratory analysis |
Palavras-chave: Microbiologia e melhoramento genético de plantas 5.01.02.03-6 toxin; C. elegans; Selection; Ricinus microbiologia e melhoramento genetico de plantas. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/11284 |
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| Texeira, Leandra Regina; UEM; Braccini, Alessandro De Lucca e; UEM; Churata, Bruno Grimaldo Martinho; UFPR; Vieira, Elisa Serra Negra; Cooperativa Central de Pesquisa Agrícola; Martins, Polyana Kelly; Cooperativa Central de Pesquisa Agrícola; Schuster, Ivan; Cooperativa Central de Pesquisa Agrícola. |
| The first step in plant transformation using somatic embryogenesis or organogenesis for plant regeneration in vitro has been the identification of genotypes with the best totipotency. Thus, the objective of this experiment was to evaluate the totipotency of ten cultivars and one soybean line from the Coodetec Gene Bank. The somatic embryos developed from immature cotyledons measuring from 4 to 6 mm in length, and the organogenesis was evaluated from mature soybean seeds. The cultivars CD 201, CD 216 and CD 220 were the most promising genotypes to produce somatic embryos, whereas the cultivar CD 217 had the highest quantity of differentiated explants, which represents the best regeneration efficiency for participating in organogenic systems. In the... |
| Tipo: Laboratory analysis |
Palavras-chave: Glycine max; Somatic embryogenesis; Organogenesis Glycine max; Somatic embryogenesis; Organogenesis Melhoramento Vegetal; Cultura de Tecidos. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12344 |
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| Çalişkan, Oguzhan; Faculty of Agriculture, University of Mustafa Kemal; Mavi, Kazim; Faculty of Agriculture, University of Mustafa Kemal; Polat, Aytekin; Faculty of Agriculture, University of Mustafa Kemal. |
| Seed germination of fig seeds is important for obtain seedlings with high variability in breeding studies, and for producing mosaic virus-free seedlings. This experiment was carried out to evaluated the effects of several treatments (priming with water for 24h, GA3 at 500 and 1000 ppm for 24h, 3% KNO3 for 24h, and stratification at 4°C for 7, 14 and 21 days) on seed germination and emergence of ‘Bursa Siyahı’ and ‘Sarılop’ fig cultivars. The application of GA3 increased the germination and emergence of these fig seeds. The highest percentages of germination and emergence were obtained with GA3 at 500 or 1000 ppm for both cultivars. The seed germination and emergence of the cultivar ‘Bursa Siyahı’ were higher than observed with the ‘Sarılop’. The... |
| Tipo: Laboratory analysis |
Palavras-chave: Ficus carica L.; Fig; Seed germination; Seedling emergence; Seed treatments seed germination and emergence Horticulture Ficus carica L.; Fig; Seed; Germination; Seedling emergence; Seed treatments seed germination and emergence. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13392 |
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| Kavand, Somayeh; Agricultural Biotechnology Research Institute of Iran; Kermani, Maryam Jafarkhani; Agricultural Biotechnology Research Institute of Iran; Haghnazari, Ali; Agricultural Biotechnology Research Institute of Iran; Khosravi, Pegah; Agricultural Biotechnology Research Institute of Iran; Azimi, Mohamad Reza; Agricultural Biotechnology Research Institute of Iran. |
| In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM... |
| Tipo: Laboratory analysis |
Palavras-chave: In vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta in vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279 |
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| Ribeiro, Luciene Oliveira; Universidade Federal de Lavras; Paiva, Luciano Vilela; Universidade Federal de Lavras; Pádua, Marlúcia Souza; Universidade Federal de Lavras; Santos, Breno Régis; Universidade Federal de Alfenas; Alves, Eduardo; Universidade Federal de Lavras; Stein, Vanessa Cristina; Universidade Federal de Goiás. |
| This work was carried out to characterise morphologically and ultrastructurally the banana callus, obtained from the scalp method. Genotypes of banana, cv. Prata anã, cultivated in vitro were used to induce meristems at the leaf base; subsequently, structures known as scalps were formed. For ultrastructural analysis, five samples of callus were collected, fixed in Karnovsky solution and analysed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The formation of three types of callus was observed: Type 1 - transparent watery callus, Type 2 - yellow callus with small clusters, Type 3 - yellow callus with large clusters. SEM analysis showed that Type 1 callus cells were elongated and that Type 2 and Type 3 callus cells were... |
| Tipo: Laboratory analysis |
Palavras-chave: 5.01.00.00-9 Agronomia 5.01.00.00-9 scanning electron microscopy; Transmission electron microscopy; Musa sp.; Cytology; Embryogenesis Agronomia. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/14501 |
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